Options for Rapid Analysis of Peptides and Proteins, Using Wide-Pore, Superficially Porous, High-Performance Liquid Chromatography Particles with Unique Bonded-Phase Ligands
نویسندگان
چکیده
منابع مشابه
Options for rapid analysis of peptides and proteins, using wide-pore, superficially porous, high-performance liquid chromatography particles with unique bonded-phase ligands.
The large size and complexity of many proteins constrains the reversed-phase high-performance liquid chromatography packings that are useful for their separation. Wide-pore, superficially porous, silica-based packings with solid 4.5-microm cores and a 0.25-microm porous outer layer (Poroshell) demonstrate a variety of characteristics that are beneficial for the separation of proteins. A shorter...
متن کاملSensitive and reproducible intact mass analysis of complex protein mixtures with superficially porous capillary reversed-phase liquid chromatography mass spectrometry.
The compatibility of superficially porous (SP) resin for label-free intact protein analysis with online capillary LC/MS is demonstrated to give improved chromatographic resolution, sensitivity, and reproducibility. The robustness of the platform was measured against several samples of varying complexity and sample loading amount. The results indicate that capillary SP columns provide high loadi...
متن کاملHIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF SULFUR MUSTARD REACTION WITH AMINO ACIDS AND PROTEINS
The interaction of sulfur mustard with aminoacids and proteins has been investigated in this study. Rats were injected with sublethal doses of sulfur mustard subcutaneously and intraperitoneally. At different time intervals, plasma and urine samples were collected. The binding affinity of sulfur mustard with urinary and plasma proteins and enzymes was studied for the first time using non-i...
متن کاملChemically Modified Eggshell Membrane as an Adsorbent for Solid-Phase-Extraction of Morphine Followed by High Performance Liquid Chromatography Analysis
In this project, eggshell membrane was used as a solid phase extraction sorbent for extraction of morphine followed by HPLC analysis. At first, raw eggshell membrane was used and then, because of low adsorption of morphine on untreated eggshell membrane (<70%), modification of eggshell membrane surface was carried out using different surfactants and amines. The amine modified eggshells characte...
متن کاملA simple, sensitive and rapid isocratic reversed-phase high-performance liquid chromatography method for determination and stability study of curcumin in pharmaceutical samples
Objective: This study was designed to develop and validate a new reversed-phase high-performance liquid chromatography (RP-HPLC) method based on Q2 (R1) International Conference on Harmonization (ICH) guideline for determination of curcumin in pharmaceutical samples. Materials and Methods: The HPLC instrument method was optimized with isocratic elution with acetonitrile: ammonium acetate (45:55...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2008
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/46.3.261